FARM-KJM5010 – Bioanalytical chemistry I: Advanced Bioanalysis for Life Sciences
Course description
Course content
The course will discuss subjects which are central for analysis of biological active substances: sample preparation (various extraction- and enrichment techniques), properties of biological sample matrices, liquid chromatography (HPLC, UPLC,?chromatographic columns, dimensioning, detectors and more), mass spectrometry (in LC-MS) and properties of various compound classes.?
Learning outcome
After completing the course:
- you have advanced knowledge of the most applied sample preparation techniques like liquid-liquid and solid-phase extraction (both off-line and on-line) together with important trends within sample preparation of biological matrices.
- you have advanced knowledge on specific topics within liquid chromatography like column types and miniaturization.
- you have good knowledge on the use of mass spectrometry in combination with liquid chromatography, included ionisation, analysis, detection and spectrum interpretation.
- you are acquainted with the practical application of sample preparation, liquid chromatography and mass spectrometry within drug analysis, therapeutic drug monitoring (TDM) and determination of drugs of abuse (forensic - and doping analysis) as well as choice of sample matrix for the various types of analyses.
- you can discuss, from a given sample matrix and the target analytes, which sample preparation-, separation- and detection technique is most logical to choose when setting up a new method
Admission to the course
The course is primarily open to students who have been admitted to the Master`s Degree Programme in Pharmacy at the Department?of Pharmacy and who have passed all courses up to and including FARM3130 – Advanced Pharmacy Practice Experience (APPE) and?have all compulsory activities approved in the courses FARM4100 – Personalised Drug Therapy, FARM4110 – Comprehensive Biopharmacy and Formulation Technology and FARM4120 – Drug Development/ passed FARM4910K – Farmakoterapi og samfunnsfarmasi med fokus p? norske terapitradisjoner og norske forhold, FARM4920K – Fra virkestoff til ferdig formulering med fokus p? avanserte legemiddelformer and FARM4930K – Kvalitetssikring ved produksjon og klinisk utpr?vning av legemidler or students who have been admitted to?the Master`s Degree? Programme in?Chemistry at the Department of Chemistry, UiO.
Chemistry students who are admitted to study programmes at UiO must each semester register which courses and exams they wish to sign up for?in Studentweb.?
Pharmacy students need to contact the Study Administration at the Department of Pharmacy,?studieinfo@farmasi.uio.no, to apply for admittance to the course and exam.
If there is space available then others may also apply to take the course as long as their academic background is adequate.?Please contact the Study Administration at the Department of Pharmacy,?studieinfo@farmasi.uio.no.
Students admitted at UiO must apply for courses in Studentweb. Students enrolled in other Master's Degree Programmes can, on application, be admitted to the course if this is cleared by their own study programme.
Nordic citizens and applicants residing in the Nordic countries may apply to take this course as a single course student.
If you are not already enrolled as a student at UiO, please see our information about admission requirements and procedures for international applicants.
Recommended previous knowledge
For chemistry students: Analytical chemistry corresponding to KJM2400 – Analytical Chemistry I and KJM3400 – Analytical Chemistry II - Separation Methods.
For pharmacy students: Analytical chemistry corresponding to FRM2010 – Quality Control of Pharmaceutical ingredients (discontinued) and FRM3030 – Use of Drugs, Part III (discontinued) or FARM2130 – Pharmaceutical Analytical Chemistry.
Overlapping courses
- 10 credits overlap with FARM-KJM9010 – Bioanalytical chemistry I: Advanced Bioanalysis for Life Science.
- 10 credits overlap with FRM-KJM5050 – Bioanalytical Chemistry I: Advanced Separation Methods and Bioanalysis (continued).
- 10 credits overlap with FRM-KJM9050 – Bioanalytical Chemistry I: Advanced Separation Methods and Bioanalysis (continued).
- 5 credits overlap with KJM5460 – Analytical chemistry. Separation methods II (discontinued).
- 5 credits overlap with KJM9460 – Analytical Chemistry, Separation Methods II (discontinued).
- 5 credits overlap with FRM5310 – Biomedical analysis (discontinued).
- 5 credits overlap with FRM9310 – Biomedical analysis (discontinued).
Teaching
Lectures, colloquia?and exursions.?Students present, together with another student or alone, a scientific publication that will be handed out during the course (compulsory).?
Excursions and discussion of realistic examples within therapeutic drug monitoring (TDM), forensic science and doping make are important parts of the course.
- ca. 25 hours of lectures
- ca 8 hours of student active teaching (colloquia, flipped classroom)
- Up to 3 excursions
The presentation must be approved before you can sit for the exam.
Read more about what applies in the event of absence from compulsory activities.
Access to teaching: A student who has completed compulsory instruction and coursework and has had these approved, is not entitled to repeat that instruction and coursework. A student who has been admitted to a course, but who has not completed compulsory instruction and coursework or had these approved, is entitled to repeat that instruction and coursework, depending on available capacity.
Compulsory participation and approved assignments are valid for 3 years.
The course may be cancelled, if the number of enrolled students are low.
Examination
Final written exam, 4 hours or 45 minutes oral exam, depending on the number of candidates taking the course.?Final exam counts 100?% towards the finale grade. The decision on the form of the exam will be made around the middle of the semester.?
This course has compulsory exercises that must be approved before you can sit the finale exam.
It will also be counted as one of the three attempts to sit the exam for this course, if you sit the exam for one of the following courses:?
- FRM-KJM5050 – Bioanalytical Chemistry I: Advanced Separation Methods and Bioanalysis (continued)
- FRM-KJM9050 – Bioanalytical Chemistry I: Advanced Separation Methods and Bioanalysis (continued)
- FARM-KJM9010 – Bioanalytical chemistry I: Advanced Bioanalysis for Life Science
Examination support material
You will be able to use the calculator in Inspera if the exam is written.
Casio FX-991EX calculators are handed out to all exam candidates at the beginning of the exam if the exam is oral.
Language of examination
The examination text is given in Norwegian. You may submit your response in Norwegian, Swedish or Danish. English on request.
Grading scale
Grades are awarded on a scale from A to F, where A is the best grade and F?is a fail. Read more about the grading system.
Resit an examination
This course offers both postponed and resit of examination. Read more:
More about examinations at UiO
- Use of sources and citations
- Special exam arrangements due to individual needs
- Withdrawal from an exam
- Illness at exams / postponed exams
- Explanation of grades and appeals
- Resitting an exam
- Cheating/attempted cheating
You will find further guides and resources at the web page on examinations at UiO.