Teaching plan

Course organizer:

Arne Klungland, Professor, Oslo Universitetssykehus, Seksjon for Molekyl?rmedisin

Lecturers:

Arne Klungland (AK), Professor, Oslo Universitetssykehus, Seksjon for Molekyl?rmedisin

Vibeke Sundvold Gjerstad (AS), Forsker, UiO, Institutt for Medisinske Basalfag, Seksjon for Anatomi

Bj?rn Dalhus (BD), Forsker, CMBN, Rikshospitalet, Seksjon for Klinisk Biokjemi

Location:

The course takes place at Rikshospitalet

Demonstrations and practice Monday ¨C Wednesday: Rom: RH B1.1012A Rikshospitalet

Lectures Monday seminar rooms A3.3063/3064/3066 (3rd floor, A-building Rikshospitalet)

Lectures Tuesday, Wednesday and Thursday A3.3067 (3rd floor, A-building Rikshospitalet).

Monday 20. August

0915 - 1000 Lecture 1. A historical view on molecular biology (AK)

1015 - 1100 Lecture 2. Journal presentation (AS)

1115 - 1200 Lecture 3. Key methods in molecular biology (AK)

1230 - 1330 Demonstrations Course overview

Pippetting, sterile technique, seeding of bacteria, transformation and calculations

1330 - 1600 Practice Control pipette volume

Dilution series of E. Coli

Transformation of E. Coli

Tuesday 21. August

0915 - 1000 Lecture 4. Epigenetics (AK)

1015 - 1100 Lecture 5. Structure biology (BD)

1015 - 1100 Lecture 6. Bioinformatics (BD)

1200 ¨C 1300 Practice Pick positive clones

Initiate overnight (ON) culture for plasmid preparation

1300 - 1600 Practice Count colonies from dilution series

Purify genomic mouse DNA from tail/ear

Genotyping of mouse by PCR

Immunocytochemistry

Wednesday 22. August

0815 - 1200 Practice Preparation of plasmid DNA from E. Coli

Prepare DNA for sequencing analysis

1230 - 1700 Practice Restriction analysis of plasmid DNA

PCR amplification of plasmid DNA

Transfect mammalian cells

Prepare gels for 2h an 2i

Analyze PCR products by gel electrophoresis

Analyze restriction products by gel electrophoresis

Thursday 23. August

0930 ¨C 1130 Demonstrations 4-5 students in each group for demonstration of

(i) Phosphoimaging

(ii) Structure analysis

(iii) Immunocytochemistry

(iv) Analyze transfected mammalian cells

1215 ¨C 1500 Summary Analyze results and DNA sequences

Summary