March 20. - 22. 2013
Montebello, Radiumhospitalet Course leader: Trond StokkeWEDNESDAY 20.03. Montebello, meetingroom 4th floor, K-building
09:00 - 10:30 Principles for FCM-Fluorescence/light scatter(Stokke) Break 10:45 - 11:30 More about flow cytometers (Stokke) 11:30 - 12:15 Histograms, cytograms and data treatment (Stokke) 12:15 - 13:00 Lunch 13:00 - 13:45 The cell cycle/analysis of DNA-histograms/ploidy/BrdU (Stokke) Coffee break 14:00 - 16:00 FCM software/DiVa/Modfit (Stokke) 14:00 - 16:00 Demo flow cytometer: Facs Vantage and LSRII (Landsverk, Dale Rein)THUESDAY 21.03 NB! In courseroom J4051, 4th floor in the Radiation building
09:00 - 10:15 Immunofluorescence I (Stokke) Break 10:30 - 11:00 DNA staining (Stokke) 11:00 - 12:15 Lab (DNA-histogram-analysis, - Stokke, Landsverk) 12:15 - 13:00 Lunch 13:00 - 13:30 Miscellaneous applications for FCM/New instrumentation (Stokke) 13:30 - 14:00 Sorting (Stokke) Break 14:15 - 16:00 Lab (Leukocyte classification / analysis - Stokke, Landsverk)FRIDAY 22.03 Montebello, meetingroom 4th floor, K-building
09:00 - 09:45 Immunofluorescence II (Stokke) Break 10:00 - 10:45 Signal transduction by flow cytometry (Myklebust) 10:45 - 11:45 Apoptosis (Stokke) 11:45 - 12:45 Lunch 12:45 - 13:45 Multiparameter flow cytometry and compensation (Rasmussen) 13:45 - 14:30 Presentation of data (Landsverk) 14:30 - 16:00 Written exam (Stokke/Landsverk)