Teaching plan

March 14. - 16. 2012

Montebello, Radiumhospitalet

Course leader: Trond Stokke

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Wednesday 14.03. Montebello, meetingroom 4th floor, K-building

09:00 - 10:30 Principles for FCM-Fluorescence/light scatter(Stokke)

Break

10:45 - 11:30 More about flow cytometers (Stokke)

11:30 - 12:15 Histograms, cytograms and data treatment (Stokke)

12:15 - 13:00 Lunch

13:00 - 13:45 Miscellaneous applications for FCM (Stokke)

Break

14:00 - 16:00 FCM software/Bryte (Stokke)

14:00 - 16:00 Demo flow cytometer: Facs Vantage and LSRII (Landsverk, Dale Rein)

Thursday 15.03 NB! In courseroom B902, 9th floor in the hospital, behind the cantine

09:00 - 10:15 The cell cycle/analysis of DNA-histograms/ploidy/BRdU (Stokke)

Break

10:30 - 11:00 DNA staining (Stokke)

11:00 - 12:15 Lab (DNA-histogram-analysis, - Stokke, Landsverk)

12:15 - 13:00 Lunch

13:00 - 13:30 Immunofluorescence I (Stokke)

13:30 - 14:00 Sorting (Stokke)

Break

14:15 - 16:00 Lab (Leukocyte classification / analysis - Stokke, Landsverk)

Friday 16.03 Montebello, meetingroom 4th floor, K-building

09:00 - 09:45 Immunofluorescence II (Stokke)

Break

10:00 - 10:45 Signal transduction by flow cytometry (Myklebust)

10:45 - 11:45 Apoptosis (Stokke)

11:45 - 12:45 Lunch

12:45 - 13:45 Multiparameter flow cytometry and compensation (Rasmussen)

13:45 - 14:30 Presentation of data (Landsverk)

14:30 - 16:00 Written exam (Stokke/Landsverk)