Course leader: Trond Stokke
Programme:
Wednesday 16/3
Location: Meetingroom on the 4th floor K-bygget (nye forskningsbygget) Radiumhospitalet, Ullernchausseen 70, Montebello
900-1030 Principles for FCM-Fluorescence/light scatter (Stokke)
Break
1045-1130 More about flow cytometers (Stokke)
1130-1215 Histograms, cytograms and data treatment (Stokke)
1215-1300 Lunch
1300-1345 Miscellaneous applications for FCM (Stokke)
Coffee break
1400-1600 FCM software/Bryte (Stokke)
1400-1600 Demo flow cytometer: FacsVantage and LSRII (Landsverk, Dale Rein)
Thursday 17/3
Location: (NB! In courseroom B902, 9.th floor in the hospital, behind the kantine):
900-1015 The cell cycle/analysis of DNA-histograms/ploidy/BrdU (Stokke)
Break
1030-1100 DNA staining (Stokke)
1100-1215 Lab (DNA-histogram-analysis, Stokke, Landsverk)
1215-1300 Lunch
1300-1330 Immunofluorescence I (Stokke)
1330-1400 Sorting (Stokke)
Break
1415-1600 Lab (Leukocyte classification/analysis, Stokke, Landsverk)
Friday 18/3
Location: Meetingroom on the 4th floor K-bygget (nye forskningsbygget) Radiumhospitalet, Ullernchausseen 70, Montebello
900- 945 Immunofluorescence II (Stokke)
Break
1000-1045 Signal transduction by flow cytometry (Myklebust)
1045-1145 Apoptosis (Stokke)
1145-1245 Lunch
1245-1345 Multiparameter flow cytometry and compensation (Rasmussen)
1345-1430 Presentation of data (Landsverk)
1430-1600 Written exam (Stokke/Landsverk)