Ultraviolet-visible spectroscopy

Curriculum: Fundamentals of modern UV-visible spectroscopy. Primer, av Tony Owen, Agilent Technologies, 2000 .

Activities: Lectures in UV-visible spectroscopy, laboratory tutorials and exercises, written reports from laboratory work.

Learning requirements: The student is expected to show:

• Basic understanding of the principles of electronic spectra of molecules.
• Basic understanding of the working principle of UV-visible spectrometers.
• Understanding of the Beer-Lambert law.
• Basic understanding of the concept of ¡±chromophores¡±.
• Ability to predict molecular absorption in the 200¨C1100 nm range qualitatively.
• Basic knowledge of solvent effects.
• Ability to record UV-visible spectra including quantitative measurements.

Evaluation: Passed/failed based upon written laboratory reports. All exercises must be completed and accepted before October 14.

Schedule:

Thursday 29 September:

• 13.15-15.00: Lecture in basic UV spectroscopy, V254.

Friday 30 September:

• 09.15-14.00: Hands on demonstration of the UV-Vis instrument. Groups of two students (1 h each group).

The laboratory will be open for exercises 09.15-17.00 Monday 3 October to Wednesday 12 October:

Lecture notes: Will be available September 29.

Laboratory exercises:

1. Basic principles - an introduction to the UV-visible spectrophotometer.

2. Study of chromophores.

3. The effect of solvents on UV-visible spectra.

4. Single component analysis.